Recent Publications

Siera Martinez, Tushar Sharma, Luke Johnson, Allen Kim, Vania Ballesteros Prieto, Hovhannes Arestakesyan, Sunisha Harish, Jewel Dias, Joseph Goldfrank, Nathan Edwards, Anelia Horvath.scSNViz: visualization and analysis of cell-specific expressed SNVs, Bioinformatics, Volume 42, Issue 2, February 2026, btag023,https://doi.org/10.1093/bioinformatics/btag023

Rivera-Gonzalez, G., Butka, E., Gonzalez, C., Mintz, R., Kleb, S.*, Josephson, V.*, Kong, W., Jindal, K., Kamimoto, K., Shook, B., Rodeheffer, M., Morris, S. Comparative single-cell lineage tracing identifies distinct adipocyte precursor dynamics in skin and inguinal fat. Cell Stem Cell. 32(8):1267-1284. 2025.

Balick, V.*, Cooper, P.*, Haas, M.*, Chatterjee, R., Hafiz, S.*, Salameh, S.*, Gohel, C., Mazumder, M., Josephson, V. *, Kleb, S. *, Khorsandi, K*., Horvath, A., Rahnavard, A., Shook, B. Fibroblast-mediated macrophage recruitment supports acute skin wound healing. Journal of Investigative Dermatology. 145(7):1781-1797.e8. 2025.

Gupta, R., Cooper, P.*, Chai, W., Mulagura, P., Lee, SM, Theocharidis, G., Sidawy, A.,Shook, B., Nguyen, B. Endothelial BII Spectrin Deletion Exacerbates Inflammation and Impairs Tissue Regeneration in Ischemic-Diabetic Skin Wound Healing. Wound Repair and Regeneration. 33(6):e70103. 2025.

Krammer L, McNeely PM, Bhuiyan U, Singleton SS, Arethiya N, Argaw A, Aggarwal V, Basuchoudhary A, Mazumder M, David J, Agrawal S, Sen S, Mazumder R.PredictMod: A Platform for Predicting Medical Intervention Outcomes and Sharing Custom ML/AI Models. Network and Systems Medicine. 2025. Vol. 1(1):57-66. DOI: 10.14293/NSM.25.1.0007

Bhuiyan U, Au CCH, Edwards N, Johnson L, Kulkarni S, Martinez K, Ranzinger R, Vijay-Shanker K, Vora J, Warner K, Tiemeyer M, Mazumder R. GlycoSiteMiner: an ML/AI-assisted literature mining-based pipeline for extracting glycosylation sites from PubMed abstracts. Glycobiology. 2025 May 22. PMID:40401984

Kim S, Mazumder R. Enhancing scientific reproducibility through automated BioCompute Object creation using Retrieval-Augmented Generation from publications. 2025. Computer Science, Computation and Language. https://doi.org/10.48550/arXiv.2409.15076

Aoki-Kinoshita KF, Lisacek F, Mazumder R, Ranzinger R, Tiemeyer M, Yamada I, Packer NH.Meeting report of the GlySpace alliance and GaLSIC symposium. Glycobiology.2025 Mar 28:cwaf019. PMID: 40156285.

Clarke DJB, Evangelista JE, Xie Z, Marino GB, Byrd AI, Maurya MR, Srinivasan S, Yu K, Petrosyan V, Roth ME, Milinkov M, King CH, Vora JK, Keeney J, Nemarich C, Khan W, Lachmann A, Ahmed N, Agris A, Pan J, Ramachandran S, Fahy E, Esquivel E,Mihajlovic A, Jevtic B, Milinovic V, Kim S, McNeely P, Wang T, Wenger E, Brown MA, Sickler A, Zhu Y, Jenkins SL, Blood PD, Taylor DM, Resnick AC, Mazumder R, Milosavljevic A, Subramaniam S, Ma’ayan A. Playbook workflow builder: Interactive construction of bioinformatics workflows. PLoS Comput Biol. 2025 Apr 3;21(4):e1012901. PMID: 40179105.

J Padilla, B-S Lee, A Kim, Y-I Park, A Bansal, and J Lee. Tumor Regulatory Effect of 15-Hydroxyprostaglandin Dehydrogenase (HPGD) in Triple-Negative Breast Cancer. International Journal of Molecular Sciences. 2025. 26(5):1912 doi:10.3390/ijms26051912. PMID:40076539, PMCID: PMC11899648.

S Mazambani, A Nguyen, J Choe, B-S Lee, JY Jeong, SY Lee, C-H Kim, J Lee, TG Oh, P Singh, H Hur, J-W Kim, and TH Kim. Targeting the glucose-insulin link in head and neck squamous cell carcinoma induces cytotoxic oxidative stress and inhibits cancer growth. Cancer Research Communication.2025 5(6):921-938doi.org/10.1158/2767-9764.CRC-23-0506. PMID: 40371988, PMCID: PMC12141995.

 B-S Lee, Y-I Park, H Liu, S G Kim, H J Kim, J-H Choi, S H Rho, J Padilla, J Roh, H G Woo, H J Seo, M H Choi, Y-J Jeong, E C. Lien, S H Mehdi, D Lee, D Yoon, C-H Kim* and J Lee. The role of 7-dehydrocholesterol in inducing ER stress and apoptosis of head and neck squamous cell carcinoma. Cancer Letters. 2025. Vol.628 doi: 10.1016/j.canlet.2025.217842. PMID: 40456399. 

N. M Dowling, G Khramtsova, O Olopade, S Samankan, B-S Lee, and J Lee. Differential BACH1 Expression in Basal-like Breast Tumors of Black Women Identified via Immunohistochemistry. Current Oncology. 2025. July 14;32(7)404. PMID:40710214. PMCID: PMC12293708. Doi:10.3390/curroncol32070404.

K-N Kim, N-S Kang, H-R Lee, S Jung, S Ju, Y-J Lee, K Lee, S Jung, J Im, and J Lee. Effects of resistant starch on metabolic markers and gut microbiota in women with metabolic syndrome risk factors: a randomized, double-blind, pilot study. Nutrients. Accepted.2025.  

Li Z, Peng Y, Chiang HC, Gao Y, Chu C, Zhang Y, Mughal MJ, Liu C, Li R, Pei H, Zhu W. AND-1 is a critical regulator of R-loop dynamics and a target to overcome endocrine resistance. Sci Adv. 2025 Aug 8;11(32): eadv2453.

Zhou S, Zhang Y, Li Z, Li Z, Latham PS, Meng Y, Chen W, Yang P, Hou C, Ma J, Zhu W. And-1 coordinates with polymerase δ to regulate nucleotide excision repair and UVB-induced skin tumorigenesis. Nat Commun. 2025 Oct 21;16(1):9313. doi: 10.1038/s41467-025-64380-4. PubMed PMID: 41120356; PubMed Central PMCID: PMC12540712.

Li Z, Peng Y, Chiang HC, Gao Y, Chu C, Zhang Y, Mughal MJ, Liu C, Li R, Pei H, Zhu W. AND-1 is a critical regulator of R-loop dynamics and a target to overcome endocrine resistance. Sci Adv. 2025 Aug 8;11(32):eadv2453. doi: 10.1126/sciadv.adv2453. Epub 2025 Aug 8. PubMed PMID: 40768568; PubMed Central PMCID: PMC12327453.

Zhou S, Zhang Y, Belmar J, Hou C, Zhang Y, Peng C, Meng Y, Li Z, Mughal MJ, Gao Y, Seto E, Shen M, Hall MD, Ma J, Ma CX, Li S, Zhu W. Stabilization of RUNX1 Induced by O-GlcNAcylation Promotes PDGF-BB-Mediated Resistance to CDK4/6 Inhibitors in Breast Cancer. Cancer Res. 2025 May 2;85(9):1708-1724. doi: 10.1158/0008-5472.CAN-24-2492. PubMed PMID: 39937190; PubMed Central PMCID: PMC12625700.

Noonepalle, S., Gracia-Hernandez, M., Aghdam, N., Berrigan, M., Coulibaly, H., Li, X., Zevallos-Delgado, C., Pletcher, A., Palmer, E., Knox, T., Sotomayor, E., Chiappinelli, K, Wardrop, D., Horvath, A., Shook, B., Lee., N., Dritschilo, A., Fernandes, R., Musunuri, K., Shibata, M., Villagra, A. Cell therapy using ex-vivo reprogrammed macrophages enhances antitumor immune responses in melanoma. Journal of Experimental and Clinical Cancer Research. 43, 263. 2024.

Cooper, P.*, Noonepalle, S.*, Babirye, N.*, Varshney, R., Rudolph, M., Dhaliwal, T.*, Nguyen, D., Shook, B. Targeting G-protein-coupled receptor 84 signaling regulates acute inflammation in normal and diabetic skin wounds. Cell Reports.43(6):114288. 2024.

Martinez K, Agirre J, Akune Y, Aoki-Kinoshita KF, Arighi C, Axelsen KB, Bolton E, Bordeleau E, Edwards NJ, Fadda E, Feizi T, Hayes C, Ives CM, Joshi HJ, Krishna Prasad K, Kossida S, Lisacek F, Liu Y, Lütteke T, Ma J, Malik A, Martin M, Mehta AY, Neelamegham S, Panneerselvam K, Ranzinger R, Ricard-Blum S, Sanou G, Shanker V, Thomas PD, Tiemeyer M, Urban J, Vita R, Vora J, Yamamoto Y, Mazumder R. Functional implications of glycans and their curation: insights from the workshop held at the 16th Annual International Biocuration Conference in Padua, Italy. Database (Oxford). 2024 Aug 13;2024:baae073. PMID: 39137905

Keeney JG, Gulzar N, Baker JB, Klempir O, Hannigan GD, Bitton DA, Maritz JM, King CHS 4th, Patel JA, Duncan P, Mazumder R. Communicating computational workflows in a regulatory environment. Drug Discov Today. 2024 Jan 12:103884. PMID: 38219969

Wu J, Singleton SS, Bhuiyan, Krammer L, Mazumder R. Multi-omics approaches to studying gastrointestinal microbiome in the context of precision medicine and machine learning. Front. Mol. Biosci., 19 January 2024. Sec. Molecular Diagnostics and Therapeutics. Volume 10 – 2023. PMID: 38313584.

Sylvetsky AC, Clement RA, Stearrett N, Issa NT, Dore FJ, Mazumder R, King CH, Hubal MJ, Walter PJ, Cai H, Sen S, Rother KI, Crandall KA. Consumption of sucralose and acesulfame-potassium containing diet soda alters the relative abundance of microbial taxa at the species level: findings of two pilot studies. Appl Physiol Nutr Metab. 2024 Jan 1;49(1):125-134. PMID: 37902107.

Caldovic L, Ahn JJ, Andricovic J, Balick VM, Brayer M, Chansky PA, Dawson T, Edwards AC, Felsen SE, Ismat K, Jagannathan SV, Mann BT, Medina JA, Morizono T, Morizono M, Salameh S, Vashist N, Williams EC, Zhou Z, Morizono H. Datamining approaches for examining the low prevalence of N-acetylglutamate synthase deficiency and understanding transcriptional regulation of urea cycle genes. J Inherit Metab Dis. 2024; 47(6):1175- -1193. doi: 10.1002/jimd.12687. PMID: 37847851; PMCID: PMC11586597.

Bramble MS, Fourcassié V, Vashist N, Roux-Dalvai F, Zhou Y, Bumoko G, Kasendue ML, Spencer D, Musasa Hanshi-Hatuhu H, Kambale-Mastaki V, Manalo RVM, Mohammed A, McIlwain DR, Cunningham G, Summar M, Boivin MJ, Caldovic L, Vilain E, Mumba-Ngoyi D, Tshala-Katumbay D, Droit A. Glutathione peroxidase 3 is a potential biomarker for konzo. Nat Commun. 2024; 15(1):7811.

Chiang HC, Qi L, Mitra P, Huang Y, Hu Y, Li R. R-loop functions in Brca1-associated mammary tumorigenesis. Proc Natl Acad Sci U S A. 2024 Aug 13;121(33):e2403600121.

Mughal MJ, Zhang Y, Li Z, Zhou S, Peng C, Zhang YQ, Seto E, Shen M, Hall MD, Zhu W. TFAP2C-DDR1 axis regulates resistance to CDK4/6 inhibitor in breast cancer. Cancer Lett. 2025 Feb 1;610:217356. doi: 10.1016/j.canlet.2024.217356. Epub 2024 Nov 26. PubMed PMID: 39603379; PubMed Central PMCID: PMC11783577.

Zhang Y, Zhou S, Kai Y, Zhang YQ, Peng C, Li Z, Mughal MJ, Julie B, Zheng X, Ma J, Ma CX, Shen M, Hall MD, Li S, Zhu W. O-GlcNAcylation of MITF regulates its activity and CDK4/6 inhibitor resistance in breast cancer. Nat Commun. 2024 Jul 3;15(1):5597. doi: 10.1038/s41467-024-49875-w. PubMed PMID: 38961064; PubMed Central PMCID: PMC11222436.

Lisacek F, Tiemeyer M, Mazumder R, Aoki-Kinoshita KF. Worldwide Glycoscience Informatics Infrastructure: The GlySpace Alliance. JACS Au. eCollection 2023 Jan 23. PMID: 36711080.

Vora J, Navelkar R, Vijay-Shanker K, Edwards N, Martinez K, Ding X, Wang T, Su P, Ross K, Lisacek F, Hayes C, Kahsay R, Ranzinger R, Tiemeyer M, Mazumder R. The glycan structure dictionary-a dictionary describing commonly used glycan structure terms. Glycobiology. 2023 Feb 17:cwad014. PMID: 36799723.

Edwards N, Dillard C, NM Prashant, Liu H, Yang M, Ulianova, E, Horvath A. (2023) SCExecute: custom cell barcode-stratified analyses of scRNA-seq data. Bioinformatics. 1;39(1):btac768. doi: 10.1093/bioinformatics/btac768. https://pubmed.ncbi.nlm.nih.gov/36448703/.

Li Z, Li Z, Cheng X, Wang S, Wang X, Ma S, Lu Z, Zhang H, Zhao W, Chen Z, Yao Y, Zhang C, Chao L, Li W, Fei T. (2023) Intrinsic RNA targeting constrains the utility of CRISPR-Cas13 systems. In press, Nature Biomedical Engineering.

Cheng X, Li Z, Shan R, Li Z, Wang S, Zhou W, Zhang H, Chao L, Peng J, Fei T, Li W. (2023) Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches. Nature Communications 2023, 14 (752). https://pubmed.ncbi.nlm.nih.gov/36765063/.

Nandula SR, Nylen ES, Sen S. (2023) Novel Therapeutics in Nonalcoholic Fatty Liver Disease: A Focus on Adult Stem Cells. Metab Syndr Relat Disord. doi: 10.1089/met.2022.0069. Epub ahead of print. PMID: 36625898. https://pubmed.ncbi.nlm.nih.gov/36625898/.

Sen S, Khosla S, Awan O, Cohen S and Gollie JM (2023) Endothelial dysfunction in autoimmune, pulmonary, and kidney systems, and exercise tolerance following SARS-CoV-2 infection. Front. Med. 10:1197061. doi: 10.3389/fmed.2023.1197061. Epub ahead of print. https://pubmed.ncbi.nlm.nih.gov/37575987/.

Ho T, Peng C, Seto E, Lin H. (2023) A Trapoxin A analog as a selective nanomolar inhibitor of HDAC11. ACS Chemical Biology https://doi.org/10.1021/acschembio.2c00840.

Lo RS, Cromie GA, Tang M, Teng K, Owens K, Sirr A, Kutz JN, Morizono H, Caldovic L, Ah Mew N, Gropman A, Dudley AM. The functional impact of 1,570 individual amino acid substitutions in human OTC. Am J Hum Genet. 2023; 110(5):863-879. doi: 10.1016/j.ajhg.2023.03.019. PMID: 37146589; PMCID: PMC10183466.

Owusu-Ansah M, Guptan N, Alindogan D, Morizono M, Caldovic L. NAGS, CPS1, and SLC25A13 (Citrin) at the Crossroads of Arginine and Pyrimidines Metabolism in Tumor Cells. Int J Mol Sci. 2023; 24(7):6754. doi: 10.3390/ijms24076754. PMID: 37047726; PMCID: PMC10094985.

Harafuji N, Yang C, Wu M, Thiruvengadam G, Gordish-Dressman H, Thompson RG, Bell PD, Rosenberg AZ, Dafinger C, Liebau MC, Bebok Z, Caldovic L, Guay-Woodford LM. Differential regulation of MYC expression by PKHD1/Pkhd1 in human and mouse kidneys:phenotypic implications for recessive polycystic kidney disease. Front Cell Dev Biol. 2023; 11:1270980. doi: 10.3389/fcell.2023.1270980. PMID: 38125876; PMCID: PMC10731465.

Yang C, Harafuji N, Caldovic L, Yu W, Boddu R, Bhattacharya S, Barseghyan H, Gordish-Dressman H, Foreman O, Bebok Z, Eicher EM,Guay-Woodford LM. Pkhd1^cyli/cyli mice have altered renal Pkhd1 mRNA processing and hormonally sensitive liver disease. J Mol Med (Berl). 2023; 101(9):1141-1151. doi: 10.1007/s00109-023-02351-2. PMID: 37584738; PMCID: PMC10482757.

Zhang YJ, Yang C, Wang W, Harafuji N, Stasiak P, Bell PD, Caldovic L, Sztul E, Guay-Woodford LM, Bebok Z. Cystin is required for maintaining fibrocystin (FPC) levels and safeguarding proteome integrity in mouse renal epithelial cells: A mechanistic connection between the kidney defects in cpk mice and human ARPKD. FASEB J. 2023; 37(7):e23008. doi: 10.1096/fj.202300100R. PMID: 37318790; PMCID:PMC10929748.

Liu J, Chiang HC, Xiong W, Griffith SC, Dȕlfer, Deng H, Sun X, Yin YW, Li W, Audoly LP, An Z, Schȕrpf T, Li R, Zhang N. A highly selective humanized DDR1 mAb

reverses immune exclusion by disrupting collagen fiber alignment in breast cancer. J Immunother Cancer. 2023 Jun;11(6): e006720.

Wu B, Qi L, Chiang HC, Pan H, Zhang X, Greenbaum A, Stark E, Wang LJ, Chen Y, Haddad BR, Clagett D, Isaacs C, Elledge R, Horvath A, Hu Y, R Li. BRCA1 deficiency in mature CD8 + T lymphocytes impairs antitumor immunity. J Immunother Cancer. 2023 Feb;11(2): e005852.

Lee J, Olivieri C, Ong C, Masterson LR, Gomez S, Lee B-S, Lorenz K, Veglia G, and Rosner MR. (2022) Raf-kinase inhibitory protein (RKIP) regulates the cAMP-dependent protein kinase signaling pathway through a positive feedback loop. Proceedings of the National Academy of Sciences of the United States of America. 119(25): e2121867119 https://pubmed.ncbi.nlm.nih.gov/35696587/.

Padilla J, Lee B-S, Zhai K, Rentz B, Bobo T, Dowling N.M, and Lee J. (2022) A heme-binding transcription factor BACH1 regulates lactate catabolism in triple-negative breast cancer. Cells. 11(7):1177 https://pubmed.ncbi.nlm.nih.gov/35406740/.

Wu B, Zhang X, Chiang H-C, Pan, H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R. (2022) RNA polymerase II pausing factor NELF in CD8 + T cells promotes antitumor immunity. Nature Communications 13:2155. https://pubmed.ncbi.nlm.nih.gov/35444206/.

Dai W, Wu F, McMyn N, Song B, Walker-Sperling V, Varriale J, Zhang H, Barouch D, Siliciano J, Li W, Siliciano R. (2022) Genome-wide CRISPR screens identify combinations of candidate latency reversal agents for targeting the latent HIV-1 reservoir. Science Translational Medicine 14 (667) abh3351.https://pubmed.ncbi.nlm.nih.gov/36260688/.

Dahlin M, Singleton SS, David JA, Basuchoudhary A, Wickström R, Mazumder R, Prast-Nielsen S. (2022) Higher levels of Bifidobacteria and tumour necrosis factor in children with drug-resistant epilepsy are associated with anti-seizure response to the ketogenic diet. eBioMedicine (part of The Lancet discovery science). PMID: 35598439. https://pubmed.ncbi.nlm.nih.gov/35598439/

Lyman DF, Bell A, Black A, Dingerdissen H, Cauley E, Gogate N, Liu D, Joseph A, Kahsay R, Crichton DJ, Mehta A, Mazumder R. (2022) Modeling and integration of N-glycan biomarkers in a comprehensive biomarker data model. Glycobiology. PMID: 35925813. https://pubmed.ncbi.nlm.nih.gov/35925813/.

Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R. RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. Nat Commun. 2022 Apr 20;13(1):2155.

Elzarki AF, Nandula SR, Awal H, Simon GL, Sen S. (2022) Cardiovascular disease (CVD) risk assessment of HIV medication regimens using hematopoietic CD34+ progenitor cells. Stem Cell Res Ther. 13(1):103. doi: 10.1186/s13287-022-02775-6. PMID: 35255964. https://pubmed.ncbi.nlm.nih.gov/35255964/

Jared M. Gollie, Sen S. (2022)  Circulating Endothelial Progenitor and Mesenchymal Stromal Cells as Biomarkers for Monitoring Disease Status and Responses to Exercise. Rev. Cardiovasc. Med. 23(12), 396.https://doi.org/10.31083/j.rcm2312396

Nandula SR, Nylen ES, Sen S. (2023) Novel Therapeutics in Nonalcoholic Fatty Liver Disease: A Focus on Adult Stem Cells. Metab Syndr Relat Disord. doi: 10.1089/met.2022.0069. Epub ahead of print. PMID: 36625898. https://pubmed.ncbi.nlm.nih.gov/36625898/

Sekhri P, Ledezma DK, Shukla A, Sweeney EE, Fernandes R. (2022) The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells Cancers, 2022, doi: 10.3390/cancers14061447. https://pubmed.ncbi.nlm.nih.gov/35326601/

Zhang, Y., J. Li, Y. Zhou, Z. Li, C. Peng, H. Pei and Zhu W. (2022) And-1 coordinates with FANCM complex to regulate Fanconi anemia signaling and cisplatin resistance. Cancer Res. 2022. Jul 22; doi: 10.1158/0008-5472.CAN-22-0769. https://pubmed.ncbi.nlm.nih.gov/35867033/

Bagchi RA, Robinson EL, Hu T, Cao J, Hong JY, Tharp CA, Qasim H, Gavin KM, da Silva JP, Major JL, McConnell BK, Seto E, Lin H, McKinsey TA. (2022) Reversible lysine fatty acylation of an anchoring protein mediates adipocyte adrenergic signaling. Proc Natl Acad Sci 119, e2119678119. https://pubmed.ncbi.nlm.nih.gov/35149557/.

Wu M, Harafuji N, O'Connor AK, Caldovic L, Guay-Woodford LM. Transcription factor Ap2b regulates the mouse autosomal recessive polycystic kidney disease genes, Pkhd1 and Cys1. Front Mol Biosci. 2023; 9:946344. doi: 10.3389/fmolb.2022.946344. PMID: 36710876; PMCID: PMC9877354.

Sun X, Wu B, Chiang HC, Deng H, Zhang X, Xiong W, Liu J, Rozeboom AM, Harris B, Blommaert E, Gomez A, Garcia RE, Zhou Y, Mitra P, Prevost M, Zhang D, Banik D,

Isaacs C, Berry D, Lai C, Chaldekas K, Latham PS, Brantner CA, Popratiloff A, Jin VX, Zhang N, Hu Y, Pujana MA, Curiel TJ, An Z, Li R. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion. Nature. 2021 Nov;599(7886):673-678. 

Yang J, Qi L, Chiang HC, Yuan B, Li R, Hu Y. BRCA1 antibodies matter. Int J Biol Sci. 2021 Jul 25;17(12):3239-3254.

Young IC, Wu B, Andricovich J, Chuang ST, Li R, Tzatsos A, Wu RC, Wu MY. (2021) Differentiation of Fetal Hematopoietic Stem Cells Requires ARID4B to Restrict Autocrine KITLG/KIT-Src Signaling. Cell Reports37(8):110036. doi: 10.1016/j.celrep.2021.110036. PMID: 34818550 PMCID: PMC8722094 https://pubmed.ncbi.nlm.nih.gov/34818550/